منابع مشابه
Synthesis of acetoacetate by liver enzymes.
Two mechanisms have been proposed for the formation of acetoacetate from acetyl-CoA by liver preparations. With the use of a partially purified system prepared from acetone powders of beef liver, Lynen et al. (1) obtained evidence that acetoacetate formation proceeded via P-hydroxy-fi-methylglutaryl coensyme A as intermediate and involved the HlMG-COAT condensing and HMG-Coh cleavage enzymes as...
متن کاملAcetoacetate Formation in Liver
In 1944, Weinhouse et al. (1) reported that the incubation of carboxyllabeled octanoate with rat liver slices yielded acetoacetate in which the carboxyl and carbonyl carbons were equally labeled. Random condensation of 2-carbon units resulting from ,8 oxidation was therefore offered by these workers as a possible mechanism for the formation of acetoacetic acid from octanoate. The conversion of ...
متن کاملThe mechanism of acetoacetate synthesis.
The observation of a coenayme A (CoA)-catalyzed acetoacetate synthesis from acetate and adenosinetriphosphate in pigeon liver extracts (1) grouped this reaction with the larger family of acetylation reactions. Furthermore it furnished partial confirmation of an earlier calculation (2) showing that 16,000 calories are required for the condensation of two acetates to acetoacetate. Thus, one energ...
متن کاملOn the mechanism of acetoacetate synthesis by guinea pig liver fractions.
1. Commercially available coenzyme A preparations were found to contain sufficient amounts of glutathione so that in the reaction with diketene significant quanties of acetoacetyl glutathione were formed. Unless allowances are made for this, significant errors in the interpretation of results can be made because liver mitochondria contain a very active acetoacetyl glutathione hydrolase. 2. Puri...
متن کاملEnzymes involved in acetoacetate formation in various bovine tissues.
1. The activities of acetoacetyl-CoA thiolase, hydroxymethylglutaryl-CoA synthase and lyase and acetoacetyl-CoA deacylase were measured in homogenates of samples of liver, rumen epithelium (long papillae), kidney and lactating mammary gland derived from slaughtered cows. 2. The activities of the four enzymes in bovine liver were similar to the activities previously reported for the correspondin...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)83962-5